Difference between revisions of "Part:BBa K4140014"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K4140030 short</partinfo>
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<partinfo>BBa_K4140014 short</partinfo>
  
 
==Part Description==
 
==Part Description==
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Glycerol-3-phosphate dehydrogenase is a novel reporter gene for E. coli, the glpD gene outperforms well-known reporter genes like lacZ and gusA. Also it has been investigated whether the glpD gene, which codes for glycerol-3-phosphate dehydrogenase, could function as an original E. coli reporter gene. In particular for transcripts with low translational efficiency, it could be demonstrated that the glpD transcript has a significantly higher stability than the lacZ transcript. It might also be demonstrated that, unlike GusA, GlpD can be selected for in vivo activity through growth on glycerol.
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Glycerol-3-phosphate dehydrogenase is a novel reporter gene for E. coli, the glpD gene outperforms well-known reporter genes like lacZ and gusA. Also, it has been investigated whether the glpD gene, which codes for glycerol-3-phosphate dehydrogenase, could function as an original E. coli reporter gene. In particular for transcripts with low translational efficiency, it could be demonstrated that the glpD transcript has significantly higher stability than the lacZ transcript. It might also be demonstrated that, unlike GusA, GlpD can be selected for in vivo activity through growth on glycerol.
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==Usage==
 
==Usage==
 
We intended to employ this novel reporter gene to analyse the activity of the promoter of our therapeutic circuit in vitro
 
We intended to employ this novel reporter gene to analyse the activity of the promoter of our therapeutic circuit in vitro
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as this reporter gene shows remarkable results by comparing it to the other reporter genes as LacZ.
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as this reporter gene shows remarkable results by comparing it to the other reporter genes such as LacZ.
  
 
==Literature Characterization==
 
==Literature Characterization==
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[[File:Glpd.png|thumb|left|Figure.1 analysis of glpD activity by comparing it to LacZ and gusA]]
 
[[File:Glpd.png|thumb|left|Figure.1 analysis of glpD activity by comparing it to LacZ and gusA]]
 
[[File:Glpd2.png|thumb|right|Figure 2. This table demonstrates the transcript level of the three reporter genes being compared]]
 
[[File:Glpd2.png|thumb|right|Figure 2. This table demonstrates the transcript level of the three reporter genes being compared]]
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
  
 
==Refrences==
 
==Refrences==
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4140030 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4140014 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4140030 parameters</partinfo>
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<partinfo>BBa_K4140014 parameters</partinfo>
 
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Latest revision as of 19:04, 11 October 2022


glpD

Part Description

Glycerol-3-phosphate dehydrogenase is a novel reporter gene for E. coli, the glpD gene outperforms well-known reporter genes like lacZ and gusA. Also, it has been investigated whether the glpD gene, which codes for glycerol-3-phosphate dehydrogenase, could function as an original E. coli reporter gene. In particular for transcripts with low translational efficiency, it could be demonstrated that the glpD transcript has significantly higher stability than the lacZ transcript. It might also be demonstrated that, unlike GusA, GlpD can be selected for in vivo activity through growth on glycerol.

Usage

We intended to employ this novel reporter gene to analyse the activity of the promoter of our therapeutic circuit in vitro as this reporter gene shows remarkable results by comparing it to the other reporter genes such as LacZ.

Literature Characterization

The two reporter genes gusA and glpD, which have better translational capacity than guaB, produced similar outcomes. The potential to select GlpD's in vivo behavior in synthetic media with glycerol as the sole carbon source is the main distinction between GusA and GlpD. This benefit of the glpD reporter gene has been effectively used to select non-canonical 5′-UTRs with high translational efficiency out of a random pool.

Figure.1 analysis of glpD activity by comparing it to LacZ and gusA
Figure 2. This table demonstrates the transcript level of the three reporter genes being compared






















Refrences

1.The art of reporter proteins in science: past, present and future applications BMB Rep., 43 (2010), pp. 451-460

2.The stability of Escherichia coli lacZ mRNA depends upon the simultaneity of its synthesis and translation EMBO J., 14 (1995), pp. 3252-3261

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 742
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 742
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 742
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 742
  • 1000
    COMPATIBLE WITH RFC[1000]