Difference between revisions of "Part:BBa K4322002"
 
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<partinfo>BBa_K4322002 short</partinfo>
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When csgA-alpha fusion chromoproteins are expressed in a culture of E. coli which is then co-cultured with cells secreting csgA-gamma(BBa K4322006), polymers of csgA-alpha-chromoprotein and csgA-gamma will form. The fibrin knob domain/alpha (BBa K4322001) and the fibrin hole domain/gamma (BBa K4322002) will interact (as they do in fibrin [1]) to form long polymers that are covalently linked to chromoproteins [2]. This part is a fusion of the csgA-alpha fusion protein (BBa_K4322005), a glycine-serine linker sequence, and the asPink chromoprotein (BBa_K1033927).
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==References:==
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[1]R. I. Litvinov et al., “Polymerization of fibrin: direct observation and quantification of individual B:b knob-hole interactions,” Blood, vol. 109, no. 1, pp. 130–138, Jan. 2007, doi: 10.1182/blood-2006-07-033910.
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[2]A. M. Duraj-Thatte et al., “Programmable microbial ink for 3D printing of living materials produced from genetically engineered protein nanofibers,” Nat Commun, vol. 12, no. 1, p. 6600, Dec. 2021, doi: 10.1038/s41467-021-26791-x.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4322002 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4322002 parameters</partinfo>
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Latest revision as of 04:00, 12 October 2022


csgAα-asPink fusion protein with glycine-serine linker


When csgA-alpha fusion chromoproteins are expressed in a culture of E. coli which is then co-cultured with cells secreting csgA-gamma(BBa K4322006), polymers of csgA-alpha-chromoprotein and csgA-gamma will form. The fibrin knob domain/alpha (BBa K4322001) and the fibrin hole domain/gamma (BBa K4322002) will interact (as they do in fibrin [1]) to form long polymers that are covalently linked to chromoproteins [2]. This part is a fusion of the csgA-alpha fusion protein (BBa_K4322005), a glycine-serine linker sequence, and the asPink chromoprotein (BBa_K1033927).


References:

[1]R. I. Litvinov et al., “Polymerization of fibrin: direct observation and quantification of individual B:b knob-hole interactions,” Blood, vol. 109, no. 1, pp. 130–138, Jan. 2007, doi: 10.1182/blood-2006-07-033910.

[2]A. M. Duraj-Thatte et al., “Programmable microbial ink for 3D printing of living materials produced from genetically engineered protein nanofibers,” Nat Commun, vol. 12, no. 1, p. 6600, Dec. 2021, doi: 10.1038/s41467-021-26791-x.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1388
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1388
    Illegal NgoMIV site found at 58
    Illegal NgoMIV site found at 1193
  • 1000
    COMPATIBLE WITH RFC[1000]