Difference between revisions of "Part:BBa K4115005"
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===Usage and Biology===
 
===Usage and Biology===
  
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The glgA sRNA can transcribe small RNA fragments that can bind specifically to glgA's mRNA assisted by the chaperone protein Hfq [https://parts.igem.org/Part:BBa_K1963000 (BBa_K1963000)] so that it can inhibit the expression of sps, which silences the sps gene in <i>S.elongatus</i> HL7942.
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The sps sRNA can transcribe small RNA fragments that can bind specifically to sps's mRNA assisted by the chaperone protein Hfq [https://parts.igem.org/Part:BBa_K1963000 (BBa_K1963000)] so that it can inhibit the expression of sps, which silences the sps gene in <i>S.elongatus</i> HL7942.
  
  

Latest revision as of 03:47, 4 October 2022


sps sRNA

An artificially synthetic small RNA(sRNA) that can bind the mRNA of sps.


Usage and Biology

The sps sRNA can transcribe small RNA fragments that can bind specifically to sps's mRNA assisted by the chaperone protein Hfq (BBa_K1963000) so that it can inhibit the expression of sps, which silences the sps gene in S.elongatus HL7942.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]