Difference between revisions of "Part:BBa K4115009"
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4115009 short</partinfo>
 
<partinfo>BBa_K4115009 short</partinfo>
  
−
An upstream homology arm from <i>S.elongatus</i> .
+
Neutral site 3(NS3) is commonly used in homologous recombination for <i>S.elongatus</i>, where NS3-1 acts as an upstream homology arm. [1]
 +
 
  
 
===Usage and Biology===
 
===Usage and Biology===
  
−
NS3-2 was used together with NS3-1 to construct plasmids required for Synechococcus.
+
It is used together with NS3-1 [https://parts.igem.org/Part:BBa_K3228025 (BBa_K3228025)] to construct plasmids required for <i>S.elongatus</i> HL7942, where NS3-2 is placed upstream of the aimed fragments.
 +
 
 +
 
  
 
<!-- -->
 
<!-- -->
Line 13: Line 16:
 
<partinfo>BBa_K4115009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4115009 SequenceAndFeatures</partinfo>
  
 +
== References ==
 +
<small>
 +
 +
[1] Niederholtmeyer H, Wolfstädter BT, Savage DF, Silver PA, Way JC. Engineering cyanobacteria to synthesize and export hydrophilic products. Appl Environ Microbiol. 2010 Jun;76(11):3462-6. doi: 10.1128/AEM.00202-10. Epub 2010 Apr 2. Erratum in: Appl Environ Microbiol. 2010 Sep;76(17):6023. PMID: 20363793; PMCID: PMC2876443.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 02:35, 4 October 2022


NS3-2

Neutral site 3(NS3) is commonly used in homologous recombination for S.elongatus, where NS3-1 acts as an upstream homology arm. [1]


Usage and Biology

It is used together with NS3-1 (BBa_K3228025) to construct plasmids required for S.elongatus HL7942, where NS3-2 is placed upstream of the aimed fragments.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Niederholtmeyer H, Wolfstädter BT, Savage DF, Silver PA, Way JC. Engineering cyanobacteria to synthesize and export hydrophilic products. Appl Environ Microbiol. 2010 Jun;76(11):3462-6. doi: 10.1128/AEM.00202-10. Epub 2010 Apr 2. Erratum in: Appl Environ Microbiol. 2010 Sep;76(17):6023. PMID: 20363793; PMCID: PMC2876443.