Difference between revisions of "Part:BBa K4380011:Design"
 
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===Design Notes===
 
===Design Notes===
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The design of this part used exploited type IIs restriction enzymes LguI ability to recognize asymmetric DNA sequences and cleave at a defined distance outside of its recognition sequence. <b>
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eGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity. eGFP (Enhanced green fluorescent protein) is used as a reporter protein to monitor the anchor peptide binding on polymer surfaces.
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When optimizing a vector sequence to use with this specific part, optimizations need to be perfomed so that LguI does not cleave DNA in any other spot, other than the plasmid and its insert.
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===Source===
 
===Source===
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''Aequorea victoria'', Zhang et al., 1996[1].
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Synthetic
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===References===
 
===References===
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[1] Zhang, G., Gurtu, V., & Kain, S. R. (1996). An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochemical and Biophysical Research Communications, 227(3), 707–711. https://doi.org/10.1006/bbrc.1996.1573

Latest revision as of 02:18, 11 October 2022


eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

eGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity. eGFP (Enhanced green fluorescent protein) is used as a reporter protein to monitor the anchor peptide binding on polymer surfaces.

Source

Aequorea victoria, Zhang et al., 1996[1].

References

[1] Zhang, G., Gurtu, V., & Kain, S. R. (1996). An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochemical and Biophysical Research Communications, 227(3), 707–711. https://doi.org/10.1006/bbrc.1996.1573