Difference between revisions of "Part:BBa K4368002"
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<partinfo>BBa_K4368002 short</partinfo>
 
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Cex is the gene coding for the exoglucanase enzyme of Celullomonas fimi. In our case, we have incorporated this enzyme into a regulated gene construct under the control of the pcstA promoter. We have also included an export site, yebF, so that this enzyme can be expressed in the cell membrane and thus function correctly together with the enzymes of the CAZymes complex. In addition, the sequence has been optimised in order to incorporate it into the Escherichia coli organism. Finally, a double terminator has been used.
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==Description ==
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''BglX'' (<partinfo>BBa_K523002</partinfo>) encodes for the β-glucosidase gene of ''Escherichia coli''. This enzyme is responsible of the degradation of cellulose working coordinated with the genes ''cenA'' and ''cex''. This complex is known as CAZymes. In detail, ''bglX'' degradates the cellobiose formed by ''cenA'' and ''cex'' and transformed it into glucose. This basic part only corresponds to the coding sequence of the gene, no RBS or terminator is attached.
  
 
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<span class='h3bb'>Sequence and Features</span>
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==<span class='h3bb'>Sequence and Features</span>==
 
<partinfo>BBa_K4368002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4368002 SequenceAndFeatures</partinfo>
  
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==Contribution ==
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*'''Group:''' [https://2022.igem.wiki/uma-malaga/index.html UMA_MALAGA]
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*'''Author:''' Molina Calvo, Alonso
  
 
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Latest revision as of 18:52, 7 October 2022

bglX

Description

BglX (BBa_K523002) encodes for the β-glucosidase gene of Escherichia coli. This enzyme is responsible of the degradation of cellulose working coordinated with the genes cenA and cex. This complex is known as CAZymes. In detail, bglX degradates the cellobiose formed by cenA and cex and transformed it into glucose. This basic part only corresponds to the coding sequence of the gene, no RBS or terminator is attached.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1297
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1207

Contribution