Difference between revisions of "Part:BBa K4115023:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Designed for constitutively expression of sf-GFP protein in E. coli. | + | Designed for constitutively expression of sf-GFP protein with degradation tag in E. coli. |
+ | After being transfected with this part, the E.ocli will have a constitutive green fluorescence. | ||
===Source=== | ===Source=== |
Latest revision as of 13:53, 3 October 2022
J23101-B0034-sfGFP(with LVA)-B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed for constitutively expression of sf-GFP protein with degradation tag in E. coli. After being transfected with this part, the E.ocli will have a constitutive green fluorescence.
Source
Obtained by assembling the corresponding parts in sequence by Gibson Assembly.