Difference between revisions of "Part:BBa K4115039:Design"

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===Design Notes===
 
===Design Notes===
  
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The function of LuxR is first verified in E.coli, which will then be used in A.caulinodans ORS571 to construct communications and regulations  between E.coli and S.elongatus.
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The function of LuxR is first verified in <i>E. coli</i>, which will then be used in <i>A.caulinodans</i> ORS571 to build a communication and regulation system between <i>E. coli</i> and <i>S.elongatus</i> HL7942.
  
 
===Source===
 
===Source===
  
−
The LuxR and sfGFP fragments were synthesized by the company. Plasmids are constructed by our team.
+
The sequence of LuxR is gained from the iGEM parts [https://parts.igem.org/Part:BBa_C0062 (BBa_C0062)]. The LuxR and sfGFP fragments were synthesized by the company. The plasmid was constructed by our team.
  
 
===References===
 
===References===

Latest revision as of 07:09, 4 October 2022


J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


Design Notes

The function of LuxR is first verified in E. coli, which will then be used in A.caulinodans ORS571 to build a communication and regulation system between E. coli and S.elongatus HL7942.

Source

The sequence of LuxR is gained from the iGEM parts (BBa_C0062). The LuxR and sfGFP fragments were synthesized by the company. The plasmid was constructed by our team.

References