Difference between revisions of "Part:BBa K4115039:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | The function of LuxR is first verified in <i>E. coli</i>, which will then be used in <i>A.caulinodans</i> ORS571 to build a communication and regulation system between <i>E. coli</i> and <i>S.elongatus</i> HL7942. | ||
===Source=== | ===Source=== | ||
− | The LuxR and sfGFP fragments were synthesized by the company. | + | The sequence of LuxR is gained from the iGEM parts [https://parts.igem.org/Part:BBa_C0062 (BBa_C0062)]. The LuxR and sfGFP fragments were synthesized by the company. The plasmid was constructed by our team. |
===References=== | ===References=== |
Latest revision as of 07:09, 4 October 2022
J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985
Design Notes
The function of LuxR is first verified in E. coli, which will then be used in A.caulinodans ORS571 to build a communication and regulation system between E. coli and S.elongatus HL7942.
Source
The sequence of LuxR is gained from the iGEM parts (BBa_C0062). The LuxR and sfGFP fragments were synthesized by the company. The plasmid was constructed by our team.