Difference between revisions of "Part:BBa K4115008"
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<partinfo>BBa_K4115008 short</partinfo> | <partinfo>BBa_K4115008 short</partinfo> | ||
− | This is a stable sequence located downstream of the nifA sequence in the genome of Azorhizobium caulinodans ORS571. It is used as a one-sided homology arm on the designed plasmid to help recombine knockout of nifA on its original genome and introduce the designed regulatory characterization sequence. | + | This is a stable sequence located downstream of the nifA sequence in the genome of Azorhizobium caulinodans ORS571. It is used as a one-sided homology arm on the designed plasmid to help recombine knockout of nifA on its original genome and introduce the designed regulatory characterization sequence.[1]<br> |
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− | < | + | |
+ | ===Usage and Biology=== | ||
+ | Detailed applications and related experiments can be found in <partinfo>BBa_K4115002</partinfo> <partinfo>BBa_K4115041</partinfo> | ||
+ | <br> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4115008 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4115008 SequenceAndFeatures</partinfo> | ||
− | + | [1]Ryu, M. H., Zhang, J., Toth, T., Khokhani, D., Geddes, B. A., Mus, F., Garcia-Costas, A., Peters, J. W., Poole, P. S., Ané, J. M., & Voigt, C. A. (2020). Control of nitrogen fixation in bacteria that associate with cereals. Nature microbiology, 5(2), 314–330. https://doi.org/10.1038/s41564-019-0631-2 | |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K4115008 parameters</partinfo> | <partinfo>BBa_K4115008 parameters</partinfo> | ||
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Latest revision as of 10:53, 4 October 2022
NifA_downstream
This is a stable sequence located downstream of the nifA sequence in the genome of Azorhizobium caulinodans ORS571. It is used as a one-sided homology arm on the designed plasmid to help recombine knockout of nifA on its original genome and introduce the designed regulatory characterization sequence.[1]
Usage and Biology
Detailed applications and related experiments can be found in BBa_K4115002 BBa_K4115041
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 138
Illegal BamHI site found at 477 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[1]Ryu, M. H., Zhang, J., Toth, T., Khokhani, D., Geddes, B. A., Mus, F., Garcia-Costas, A., Peters, J. W., Poole, P. S., Ané, J. M., & Voigt, C. A. (2020). Control of nitrogen fixation in bacteria that associate with cereals. Nature microbiology, 5(2), 314–330. https://doi.org/10.1038/s41564-019-0631-2