Difference between revisions of "Part:BBa K4387999:Design"
 
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===Design Notes===
 
===Design Notes===
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No codon optimization was necessary. The sequence was taken directly from the reference paper.
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No codon optimization was necessary. HlyB is found in the composite part <partinfo>BBa_K4387987</partinfo>, allowing the constitutive expression of the HlyA secretion machinery.
  
  
  
 
===Source===
 
===Source===
−
 
 
Sequence of the HlyB was obtain from the paper of Ruano-Gallego et al. [1]
 
Sequence of the HlyB was obtain from the paper of Ruano-Gallego et al. [1]
 +
 +
  
 
===References===
 
===References===
 
* [1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0
 
* [1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0
−
* [2]
 
−
* [3]
 

Latest revision as of 10:38, 6 October 2022


HlyB of the hemolysin A one-step secretion system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1096
    Illegal AgeI site found at 1266
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No codon optimization was necessary. HlyB is found in the composite part BBa_K4387987, allowing the constitutive expression of the HlyA secretion machinery.


Source

Sequence of the HlyB was obtain from the paper of Ruano-Gallego et al. [1]


References

  • [1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0