Difference between revisions of "Part:BBa K4324002:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
N/A
+
A nucleotide sequence was found from the protein sequence, for expression in E. coli.
  
 +
===Source===
  
 +
This original protein sequence was taken from the '''xylB gene''' of '''Escherichia coli ''' (strain K12), which can be found on the [https://rest.uniprot.org/uniprotkb/P09099.fasta Uniprot database].
  
−
===Source===
+
Alternatively, the protein sequence is pasted below:
  
−
xylB gene from Escherichia coli (strain K12)
+
<code>
 +
MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQDPEQWWQATDRAMKALG
 +
DQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCAQECTLLEARVPQSRVITG
 +
NLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRMTGEFASDMSDAAGTMWLDVA
 +
KRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAKAWGMATVPVVAGGGDNAAGAVG
 +
VGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHSFCHALPQRWHLMSVMLSAASCLDW
 +
AAKLTGLSNVPALIAAAQQADESAEPVWFLPYLSGERTPHNNPQAKGVFFGLTHQHGPNE
 +
LARAVLEGVGYALADGMDVVHACGIKPQSVTLIGGGARSEYWRQMLADISGQQLDYRTGG
 +
DVGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAYQPRRETFRRLYQQLL<br>
 +
PLMA
 +
</code>
  
 
===References===
 
===References===
 +
1. https://www.uniprot.org/uniprotkb/P09099/entry<br>

Latest revision as of 14:15, 12 October 2022


lacPO-RBS-XK-T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 719
    Illegal AgeI site found at 1007
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A nucleotide sequence was found from the protein sequence, for expression in E. coli.

Source

This original protein sequence was taken from the xylB gene of Escherichia coli (strain K12), which can be found on the Uniprot database.

Alternatively, the protein sequence is pasted below:

MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQDPEQWWQATDRAMKALG DQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCAQECTLLEARVPQSRVITG NLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRMTGEFASDMSDAAGTMWLDVA KRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAKAWGMATVPVVAGGGDNAAGAVG VGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHSFCHALPQRWHLMSVMLSAASCLDW AAKLTGLSNVPALIAAAQQADESAEPVWFLPYLSGERTPHNNPQAKGVFFGLTHQHGPNE LARAVLEGVGYALADGMDVVHACGIKPQSVTLIGGGARSEYWRQMLADISGQQLDYRTGG DVGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAYQPRRETFRRLYQQLL
PLMA

References

1. https://www.uniprot.org/uniprotkb/P09099/entry