Difference between revisions of "Part:BBa K4165195"
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This part encodes a part of the Amyloid 𝛽 fragment (34-42) which has the ability to bind to A𝛽 plaques inside the brain.
 
This part encodes a part of the Amyloid 𝛽 fragment (34-42) which has the ability to bind to A𝛽 plaques inside the brain.
  
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===Usage and Biology===
 
===Usage and Biology===
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Segments of amyloid beta fibrils are widely used as a recognition sequence for amyloid beta plaques inside the brain, this is due to the homotypic interactions in the C-terminus of fibrils. This peptide starts from amino acid 29 to 42 of the fibril. This binding peptide starts from amino acid 34 to 42.
  
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===<span class='h3bb'>Sequence and Features</span>===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4165195 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165195 SequenceAndFeatures</partinfo>
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===Dry lab===
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<p style=" font-weight: bold; font-size:14px;"> Modeling </p>
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The peptide was modeled by several software (Alphafold - Apptest - Pepfold) and the best model was obtained from Apptest.
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/ab-34-42-model.png" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                                Figure 1.: Amyloid-beta (34-42) top model visualized by pymol.
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===References===
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1-Zhao, Y., Cai, J., Liu, Z., Li, Y., Zheng, C., Zheng, Y., ... & Liu, Y. (2018). Nanocomposites inhibit the formation, mitigate the neurotoxicity, and facilitate the removal of β-amyloid aggregates in Alzheimer’s disease mice. Nano letters, 19(2), 674-683.
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Latest revision as of 19:55, 11 October 2022


Amyloid beta peptide 15 (Aβ 34-42)

This part encodes a part of the Amyloid 𝛽 fragment (34-42) which has the ability to bind to A𝛽 plaques inside the brain.


Usage and Biology

Segments of amyloid beta fibrils are widely used as a recognition sequence for amyloid beta plaques inside the brain, this is due to the homotypic interactions in the C-terminus of fibrils. This peptide starts from amino acid 29 to 42 of the fibril. This binding peptide starts from amino acid 34 to 42.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Dry lab

Modeling

The peptide was modeled by several software (Alphafold - Apptest - Pepfold) and the best model was obtained from Apptest. 

                               Figure 1.: Amyloid-beta (34-42) top model visualized by pymol.

References

1-Zhao, Y., Cai, J., Liu, Z., Li, Y., Zheng, C., Zheng, Y., ... & Liu, Y. (2018). Nanocomposites inhibit the formation, mitigate the neurotoxicity, and facilitate the removal of β-amyloid aggregates in Alzheimer’s disease mice. Nano letters, 19(2), 674-683.