Difference between revisions of "Part:BBa K4201012"

Latest revision as of 01:08, 12 October 2022

RUBY

Chimeric expression of RUBY reporter in G. max allows visualization of an genomically integrated construct.

RUBY is a reporter construct that is composed of the enzymes that are needed for the biosynthesis of betalain¹. Betalain is a naturally occurring compound found in Caryophyllales and it produces a bright red color². When used as a reporter, it will turn the transgenic plant tissue red and it does not need UV light to observe like a GFP reporter would, as seen in the figure below. The RUBY reporter allows for the survival of the modified plant unlike a GUS reporter, which is lethal to the plant.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3745
Illegal BamHI site found at 2394
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 330
Illegal NgoMIV site found at 909
Illegal NgoMIV site found at 2622
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 3968

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4201012 short</partinfo>
-Description
+[[File:RUBY visualization G max.png|200px|thumb|right|Chimeric expression of RUBY reporter in G. max allows visualization of an genomically integrated construct. ]]
+RUBY is a reporter construct that is composed of the enzymes that are needed for the biosynthesis of betalain<sup>1</sup>. Betalain is a naturally occurring compound found in Caryophyllales and it produces a bright red color<sup>2</sup>. When used as a reporter, it will turn the transgenic plant tissue red and it does not need UV light to observe like a GFP reporter would, as seen in the figure below. The RUBY reporter allows for the survival of the modified plant unlike a GUS reporter, which is lethal to the plant.
 <!-- Add more about the biology of this part here