Difference between revisions of "Part:BBa K4201010"
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| − | + | The 𝛃-Glucuronidase (GUS) gene from <i>Escherichia coli</i> has been used as a reporter gene in many different plant species for many years<sup>1</sup>. It produces a blue color that is expressed when the gene is successfully integrated into the plant genome; its expression can be quantified by fluorometric and spectrophotometric analysis. However, assays with GUS require the plant to be destroyed in order to be analyzed. Therefore, we did not actually use this gene as part of our final construct, as we would no longer be able to actually grow the plant after confirmation that our construct was integrated into the genome. | |
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Latest revision as of 02:21, 12 October 2022
𝛃-Glucuronidase (GUS)
The 𝛃-Glucuronidase (GUS) gene from Escherichia coli has been used as a reporter gene in many different plant species for many years1. It produces a blue color that is expressed when the gene is successfully integrated into the plant genome; its expression can be quantified by fluorometric and spectrophotometric analysis. However, assays with GUS require the plant to be destroyed in order to be analyzed. Therefore, we did not actually use this gene as part of our final construct, as we would no longer be able to actually grow the plant after confirmation that our construct was integrated into the genome.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 2018