Difference between revisions of "Part:BBa K4201003"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4201003 short</partinfo>
 
<partinfo>BBa_K4201003 short</partinfo>
  
−
description
+
CrtE is the gene which encodes geranylgeranyl diphosphate synthase, an essential enzyme in the MVA pathway in plants, through which paclitaxel is eventually synthesized<sup>1</sup>. After GPP is converted into FPP, geranylgeranyl diphosphate synthase converts it into GGPP, an important precursor in the MVA pathway<sup>2</sup>. We are using this gene as part of our construct to ensure that paclitaxel is synthesized in the cytosol rather than through the MEP pathway in the chloroplast, which would complicate our project by requiring additional export tags.
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:38, 11 October 2022


CrtE

CrtE is the gene which encodes geranylgeranyl diphosphate synthase, an essential enzyme in the MVA pathway in plants, through which paclitaxel is eventually synthesized1. After GPP is converted into FPP, geranylgeranyl diphosphate synthase converts it into GGPP, an important precursor in the MVA pathway2. We are using this gene as part of our construct to ensure that paclitaxel is synthesized in the cytosol rather than through the MEP pathway in the chloroplast, which would complicate our project by requiring additional export tags.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 926