Difference between revisions of "Part:BBa K4385012:Design"

 
 
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===Design Notes===
 
===Design Notes===
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This part is composed of ZraSR two-component signal transport system, PzraP and OmpC-ZBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PzraP promoter promotes the transcription of downstream OmpC-ZBP, and realizes the adsorption of zinc ions in the environment. Add 6*Hig Tag to this sequence to purify recombinant protein.
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The copper sensor part is composed of PcusC and GFP. The initiation of the copper-inducible promoter PcusC requires the support of the CusSR two-component membrane associated sensor kinase system, which is present in the strain E. coli XL-1 Blue.
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===Source===
 
===Source===

Latest revision as of 14:22, 6 October 2022


Copper sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 826


Design Notes

The copper sensor part is composed of PcusC and GFP. The initiation of the copper-inducible promoter PcusC requires the support of the CusSR two-component membrane associated sensor kinase system, which is present in the strain E. coli XL-1 Blue.

Source

It comes from E. coli genomic DNA.

References