Difference between revisions of "Part:BBa K4488024:Design"

 
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===Design Notes===
 
===Design Notes===
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Edits have been made to remove restriction sites and optimise for cloning into the plasmid vector for E. coli.  
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Cytosine at position 153 was changed to thymine and thymine at position 256 was changed to guanine. No restriction sites needed to be removed.
  
  
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===Source===
 
===Source===
  
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BBa_K1321014
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The sequence was adapted from the 2014 Imperial team (BBa_K1321014).
  
 
===References===
 
===References===

Latest revision as of 04:13, 9 October 2022


Modified cellulose binding domain from Clostridium thermocellum cellulosomal scaffold protein A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cytosine at position 153 was changed to thymine and thymine at position 256 was changed to guanine. No restriction sites needed to be removed.


Source

The sequence was adapted from the 2014 Imperial team (BBa_K1321014).

References