Difference between revisions of "Part:BBa K200009"

Latest revision as of 21:38, 19 October 2009

DpnII Composite

This part encodes a ribosome binding site and DpnII coding region (from BBa_K200015).

Detail

This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37°C.

DpnII is a 4 base cutter, leaving a 4 base overhang.

The cutting site is as shown:

Usage and Biology

Enzyme Properties can be viewed at the [http://www.neb.com/nebecomm/products/productR0543.asp DpnII Datasheet] from New England Biolabs

The gene was used alongside with the restriction enzyme TaqI and the methylase Dam by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project as part of the genome deletion module.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[http://www.neb.com/nebecomm/products/productR0543.asp Information from NEB]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K200009 short</partinfo>
+<br><br>
-This sequence codes for the restriction enzyme DpnII.<br>
+This part encodes a ribosome binding site and DpnII coding region (from [https://parts.igem.org/Part:BBa_K200015 BBa_K200015]).<br>
+<br>
-This is a Type II restriction enzyme that recognises the sequence GATC.  Its activity can be blocked by dam methylation.
+===Detail===
+This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.'  Its activity is blocked by dam methylation.
 The working temperature is 37°C.
-As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.
+DpnII is a 4 base cutter, leaving a 4 base overhang.
+<br>
+The cutting site is as shown:
+<br><br>
+[[Image:II09_DpnII.png]]
+<br><br>
 ===Usage and Biology===
-DpnII is a 4 base cutter, leaving a 4 base overhang.
+Enzyme Properties can be viewed at the [http://www.neb.com/nebecomm/products/productR0543.asp DpnII Datasheet] from New England Biolabs
-<br>
-The cutting site is as shown:
-<br>
-NGATCN  ->  N / GATCN <br>
-NCTAGN  ->  NCTAG / N
+The gene was used alongside with the restriction enzyme TaqI and the methylase Dam by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project as part of the genome deletion module.
 <span class='h3bb'>Sequence and Features</span>
+===Sequence and Features===
 <partinfo>BBa_K200009 SequenceAndFeatures</partinfo>