Difference between revisions of "Part:BBa K4169024"
Ruixuan Zhu (Talk | contribs) |
|||
(3 intermediate revisions by the same user not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K4169024 short</partinfo> | <partinfo>BBa_K4169024 short</partinfo> | ||
− | Two loxP sites are on the same DNA strand and in opposite directions. And after expressing, it'll produce Cyclic recombinase which mediates sequence | + | Two loxP sites are on the same DNA strand and in opposite directions. And after expressing, it'll produce Cyclic recombinase which mediates sequence flip between reverse loxPs. |
− | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | When we express the Cre which recognizes the reverse repeat sequence at both ends of the loxP site and binds to form a dimer, which then binds to a dimer at another loxP site to form a tetramer.This allows us to have our engineered bacteria flip the gene between the two reverse loxP sites. | |
<!-- --> | <!-- --> | ||
+ | ===Sequence and Features=== | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4169024 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4169024 SequenceAndFeatures</partinfo> |
Latest revision as of 12:19, 12 October 2022
produce Cre enzyme
Two loxP sites are on the same DNA strand and in opposite directions. And after expressing, it'll produce Cyclic recombinase which mediates sequence flip between reverse loxPs.
Usage and Biology
When we express the Cre which recognizes the reverse repeat sequence at both ends of the loxP site and binds to form a dimer, which then binds to a dimer at another loxP site to form a tetramer.This allows us to have our engineered bacteria flip the gene between the two reverse loxP sites.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 356
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 42
- 1000COMPATIBLE WITH RFC[1000]