Difference between revisions of "Part:BBa K4165111"

 
 
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<partinfo>BBa_K4165111 short</partinfo>
 
<partinfo>BBa_K4165111 short</partinfo>
  
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part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
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Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with IC<sub>50</sub> of 0.9 ± 0.1.
  
 
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<span class='h3bb'>Sequence and Features</span>
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===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K4165111 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165111 SequenceAndFeatures</partinfo>
  
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===Dry Lab Characterization===
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<p style=" font-weight: bold; font-size:14px;"> Modeling </p>
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Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
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<html>
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<style>
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table, th, td {
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  border:1px solid black; margin-left:auto;margin-right:auto;
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}
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</style>
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<body>
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<table style="width:65%">
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<table>
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  <tr>
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    <th>cbeta_deviations</th>
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    <th>clashscore</th>
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    <th>molprobity</th>
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    <th>ramachandran_favored</th>
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    <th>ramachandran_outliers</th>
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    <th>Qmean_4</th>
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    <th>Qmean_6</th>
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  </tr>
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  <tr>
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    <td>0</td>
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    <td>30.53</td>
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    <td>3.76</td>
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    <td>60</td>
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    <td>40</td>
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    <td>-6.45183</td>
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    <td>-5.513969</td>
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  </tr>
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</table>
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</body>
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</html>
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1q.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                        Figure 1.: 3D Model of H1Q peptide Modelled by AlphaFold2.0 displayed in Pymol.
  
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<!-- Uncomment this to enable Functional Parameter display
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===References===
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===Functional Parameters===
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Runyon, S. T., Zhang, Y., Appleton, B. A., Sazinsky, S. L., Wu, P., Pan, B., ... & Sidhu, S. S. (2007). Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3. Protein Science, 16(11), 2454-2471.
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<partinfo>BBa_K4165111 parameters</partinfo>
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Latest revision as of 09:35, 13 October 2022


HTRA Binding Peptide 17

Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with IC50 of 0.9 ± 0.1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Dry Lab Characterization

Modeling

Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0


cbeta_deviations clashscore molprobity ramachandran_favored ramachandran_outliers Qmean_4 Qmean_6
0 30.53 3.76 60 40 -6.45183 -5.513969 



                        Figure 1.: 3D Model of H1Q peptide Modelled by AlphaFold2.0 displayed in Pymol.

References

Runyon, S. T., Zhang, Y., Appleton, B. A., Sazinsky, S. L., Wu, P., Pan, B., ... & Sidhu, S. S. (2007). Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3. Protein Science, 16(11), 2454-2471.