Difference between revisions of "Part:BBa K4165109"
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<partinfo>BBa_K4165109 short</partinfo> | <partinfo>BBa_K4165109 short</partinfo> | ||
− | + | Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Decameric peptide, Binds specifically to PDZ domain of HTRA1 Protein with ΔG of 10.3 ± 0.2 kcal/mol. | ||
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− | <span class='h3bb'>Sequence and Features</span> | + | ===<span class='h3bb'>Sequence and Features</span>=== |
<partinfo>BBa_K4165109 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4165109 SequenceAndFeatures</partinfo> | ||
+ | ===Dry Lab Characterization=== | ||
+ | <p style=" font-weight: bold; font-size:14px;"> Modeling </p> | ||
+ | Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0 | ||
+ | |||
+ | |||
+ | <html> | ||
+ | <style> | ||
+ | table, th, td { | ||
+ | border:1px solid black; margin-left:auto;margin-right:auto; | ||
+ | } | ||
+ | </style> | ||
+ | <body> | ||
+ | <table style="width:65%"> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <th>cbeta_deviations</th> | ||
+ | <th>clashscore</th> | ||
+ | <th>molprobity</th> | ||
+ | <th>ramachandran_favored</th> | ||
+ | <th>ramachandran_outliers</th> | ||
+ | <th>Qmean_4</th> | ||
+ | <th>Qmean_6</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0</td> | ||
+ | <td>17.86</td> | ||
+ | <td>4</td> | ||
+ | <td>62.5</td> | ||
+ | <td>12.5</td> | ||
+ | <td>-6.45183</td> | ||
+ | <td>-5.513969</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </body> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | <html> | ||
+ | <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1o.jpg" style="margin-left:250px;" alt="" width="500" /></p> | ||
+ | </html> | ||
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+ | |||
+ | Figure 1.: 3D Model of H1O peptide Modelled by AlphaFold2.0 displayed in Pymol. | ||
+ | |||
+ | ===References=== | ||
+ | Rey, J., Breiden, M., Lux, V., Bluemke, A., Steindel, M., Ripkens, K., ... & Ehrmann, M. (2022). An allosteric HTRA1-calpain 2 complex with restricted activation profile. Proceedings of the National Academy of Sciences, 119(14), e2113520119. | ||
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<partinfo>BBa_K4165109 parameters</partinfo> | <partinfo>BBa_K4165109 parameters</partinfo> | ||
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Latest revision as of 09:39, 13 October 2022
HTRA Binding Peptide 15
Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
Usage and Biology
Decameric peptide, Binds specifically to PDZ domain of HTRA1 Protein with ΔG of 10.3 ± 0.2 kcal/mol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Dry Lab Characterization
Modeling
Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
cbeta_deviations | clashscore | molprobity | ramachandran_favored | ramachandran_outliers | Qmean_4 | Qmean_6 |
---|---|---|---|---|---|---|
0 | 17.86 | 4 | 62.5 | 12.5 | -6.45183 | -5.513969 |
Figure 1.: 3D Model of H1O peptide Modelled by AlphaFold2.0 displayed in Pymol.
References
Rey, J., Breiden, M., Lux, V., Bluemke, A., Steindel, M., Ripkens, K., ... & Ehrmann, M. (2022). An allosteric HTRA1-calpain 2 complex with restricted activation profile. Proceedings of the National Academy of Sciences, 119(14), e2113520119.