Difference between revisions of "Part:BBa K200001"

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*[http://en.wikipedia.org/wiki/Mismatch_repair DNA mismatch repair]
 
*[http://en.wikipedia.org/wiki/Mismatch_repair DNA mismatch repair]
 
*Regulation of protein expression.  
 
*Regulation of protein expression.  
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In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes DpnII ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K200009 BBa_K200009]) and TaqI ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K200009 BBa_K200010]).
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In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes [[Part:BBa_K200009 |DpnII]] and [[Part:BBa_K200010 |TaqI]].
  
 
[[Image:II09_dam.jpg|400px|centre|<i>Figure 1:</i> Action of Dam methylase (source: <cite>#Dam1</cite>)]]
 
[[Image:II09_dam.jpg|400px|centre|<i>Figure 1:</i> Action of Dam methylase (source: <cite>#Dam1</cite>)]]
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<centre>Figure: Action of Dam methylase (source: <cite>#Dam1</cite></centre>
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<center>Figure: Action of Dam methylase (source: <cite>#Dam1</cite>)</center>
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The gene was used alongside with the restriction enzymes [[Part:BBa_K200010 |TaqI]] and the [[Part:BBa_K200009 |DpnII]] by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project as part of the genome deletion module. The Dam (methylase) is used to prevent genome restriction due to basal expression of the restriction enzymes.
  
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===Sequence and features===
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K200001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K200001 SequenceAndFeatures</partinfo>
  
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===References===
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<biblio>#Dam1 http://fangman-brewer.genetics.washington.edu/hemimethylation.html</biblio>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 14:23, 29 September 2009

Dam methylase -> Dam

The methylase encoded by the dam gene ([http://en.wikipedia.org/wiki/Dam_(methylase) Dam methylase]) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC. When methylation occurs in the recognition site of a particular group of restriction endonuclease including MboI, this protects the DNA from cleavage.

Usage and Biology

DAM methylase (DNA adenine methylase) adds a methyl group to the adenine of the sequence 5'-GATC-3'in newly synthesized DNA. Dam methylases are important for several biological functions:

  • [http://en.wikipedia.org/wiki/DNA_replication DNA replication]
  • [http://en.wikipedia.org/wiki/Mismatch_repair DNA mismatch repair]
  • Regulation of protein expression.

In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes DpnII and TaqI.

Figure 1: Action of Dam methylase (source: #Dam1)
Figure: Action of Dam methylase (source: #Dam1)


The gene was used alongside with the restriction enzymes TaqI and the DpnII by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project as part of the genome deletion module. The Dam (methylase) is used to prevent genome restriction due to basal expression of the restriction enzymes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

<biblio>#Dam1 http://fangman-brewer.genetics.washington.edu/hemimethylation.html</biblio>