Difference between revisions of "Part:BBa K4165090"

 
 
(7 intermediate revisions by 4 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4165090 short</partinfo>
 
<partinfo>BBa_K4165090 short</partinfo>
  
−
A serine protease inhibitor is used to inhibit the action of HTRA1
+
This basic part encodes Human serine protease inhibitor WAP-four disulfide core domain 6 which is able to inhibit HtrA1 (BBa_K4165004).
  
  
−
 
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
This type of family encodes for a type of inhibitor that contains a motif which has 8 cysteine residues capable of forming four disulfide bonds at the core of the protease, thus inhibiting its action. This type of inhibitor is very effective and has high affinity for trypsin-like proteases (serine proteases), and in our case it would act as an inhibitor for the trypsin-like catalytic domain of serine protease HtrA1<sup>[1-3]</sup>.
 +
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K4165090 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165090 SequenceAndFeatures</partinfo>
  
  
−
<!-- Uncomment this to enable Functional Parameter display
 
 
===Functional Parameters===
 
===Functional Parameters===
 +
 +
 +
<html>
 +
<style>
 +
table, th, td {
 +
  border:1px solid black; margin-left:auto;margin-right:auto;
 +
}
 +
</style>
 +
<body>
 +
<table style="width:65%">
 +
<table>
 +
  <tr>
 +
    <th>GC Content%</th>
 +
    <th>Isoelectric point (PI)</th>
 +
    <th>Charge at pH 7</th>
 +
    <th>Molecular Weight (Protein)</th>
 +
  </tr>
 +
  <tr>
 +
    <td>63.6%</td>
 +
    <td>7.981</td>
 +
    <td>4.57</td>
 +
    <td>14.626</td>
 +
  </tr>
 +
</table>
 +
</body>
 +
</html>
 +
 +
 +
===Modeling===
 +
The predicted structure (AlphaFold2) is presented.
 +
 +
 +
<html>
 +
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/switches/11-alphafold.png" style="margin-left:200px;" alt="" width="500" /></p>
 +
</html>
 +
 +
                  Figure 1.: A graphical illustration showing the structure of the inhibitor.
 +
 +
===References===
 +
1. Clauss, A., Lilja, H., & Lundwall, Å. (2005). The evolution of a genetic locus encoding small serine proteinase inhibitors. Biochemical and biophysical research communications, 333(2), 383-389.<br>
 +
2. Eigenbrot, C., Ultsch, M., Lipari, M. T., Moran, P., Lin, S. J., Ganesan, R., ... & Kirchhofer, D. (2012). Structural and functional analysis of HtrA1 and its subdomains. Structure, 20(6), 1040-1050.<br>
 +
3. Grau, S., Baldi, A., Bussani, R., Tian, X., Stefanescu, R., Przybylski, M., ... & Ehrmann, M. (2005). Implications of the serine protease HtrA1 in amyloid precursor protein processing. Proceedings of the National Academy of Sciences, 102(17), 6021-6026.<br>
 +
 +
 
<partinfo>BBa_K4165090 parameters</partinfo>
 
<partinfo>BBa_K4165090 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 12:08, 13 October 2022


WAP-four disulfide core domain 6 serine protease inhibitor.

This basic part encodes Human serine protease inhibitor WAP-four disulfide core domain 6 which is able to inhibit HtrA1 (BBa_K4165004).


Usage and Biology

This type of family encodes for a type of inhibitor that contains a motif which has 8 cysteine residues capable of forming four disulfide bonds at the core of the protease, thus inhibiting its action. This type of inhibitor is very effective and has high affinity for trypsin-like proteases (serine proteases), and in our case it would act as an inhibitor for the trypsin-like catalytic domain of serine protease HtrA1[1-3].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 252
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

GC Content% Isoelectric point (PI) Charge at pH 7 Molecular Weight (Protein)
63.6% 7.981 4.57 14.626


Modeling

The predicted structure (AlphaFold2) is presented. 


                 Figure 1.: A graphical illustration showing the structure of the inhibitor.

References

1. Clauss, A., Lilja, H., & Lundwall, Å. (2005). The evolution of a genetic locus encoding small serine proteinase inhibitors. Biochemical and biophysical research communications, 333(2), 383-389.
2. Eigenbrot, C., Ultsch, M., Lipari, M. T., Moran, P., Lin, S. J., Ganesan, R., ... & Kirchhofer, D. (2012). Structural and functional analysis of HtrA1 and its subdomains. Structure, 20(6), 1040-1050.
3. Grau, S., Baldi, A., Bussani, R., Tian, X., Stefanescu, R., Przybylski, M., ... & Ehrmann, M. (2005). Implications of the serine protease HtrA1 in amyloid precursor protein processing. Proceedings of the National Academy of Sciences, 102(17), 6021-6026.