Difference between revisions of "Part:BBa K4452010"

 
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pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)  
 
pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)  
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Bacterial Resistance: Spectinomycin
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<p>Bacterial Resistance: Spectinomycin </p>
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GoldenBraid compatibility: enzyme in BsmBI, enzyme out BsaI.
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<p>GoldenBraid compatibility: enzyme in BsmBI, enzyme out BsaI.</p>
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 16:26, 9 October 2022


pLX-B3Ω1

pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)

Bacterial Resistance: Spectinomycin

GoldenBraid compatibility: enzyme in BsmBI, enzyme out BsaI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 233
    Illegal PstI site found at 622
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 616
    Illegal SpeI site found at 233
    Illegal PstI site found at 622
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 233
    Illegal PstI site found at 622
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 233
    Illegal PstI site found at 622
    Illegal NgoMIV site found at 954
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 253
    Illegal BsaI.rc site found at 596