Difference between revisions of "Part:BBa K4307026"

 
m
 
(2 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4307026 short</partinfo>
 
<partinfo>BBa_K4307026 short</partinfo>
  
waiting
+
This part is the transcription factor of Affi-PmrB/A system(BBa_K4307042), which is modified from the PmrB/A two-component system in <i>E.coli</i>. It is phosphorylated by AffipmrB, then it can activate PmrC promoter.
 +
 
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 9: Line 11:
  
 
<!-- -->
 
<!-- -->
<span class='h3bb'>Sequence and Features</span>
+
<h2>Sequence and Features</h2>
 
<partinfo>BBa_K4307026 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4307026 SequenceAndFeatures</partinfo>
  
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K4307000 parameters</partinfo>
 +
<!-- -->
 +
<html>
 +
 +
<h2>Characterization </h2>
 +
 +
<p>The following figure demonstrates our successful construction.</p>
 +
 +
<br>
 +
<div class="center">
 +
    <div class="thumb tnone">
 +
        <div class="thumbinner" style="width:50%;">
 +
            <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307026-gel.png" class="image">
 +
                <img alt="" src="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307026-gel.png" width="100%" height=auto class="thumbimage" /></a>                  <div class="thumbcaption">
 +
                <div class="magnify">
 +
                    <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307026-gel.png" class="internal" title="Enlarge"></a>
 +
                </div>
 +
                <b>Figure 1: </b> <b>The construction results of PmrA.</b>
 +
               
 +
            </div>
 +
        </div>
 +
    </div>
 +
</div>
 +
</html>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 16:53, 13 October 2022


PmrA

This part is the transcription factor of Affi-PmrB/A system(BBa_K4307042), which is modified from the PmrB/A two-component system in E.coli. It is phosphorylated by AffipmrB, then it can activate PmrC promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


Figure 1: The construction results of PmrA.