Difference between revisions of "Part:BBa K4307026"

 
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This part is the transcription factor of Affi-PmrB/A system（BBa_K4307042）, which is modified from the PmrB/A two-component system in <i>E.coli</i>. It is phosphorylated by AffipmrB, then it can activate PmrC promoter.
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<span class='h3bb'>Sequence and Features</span>
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<h2>Sequence and Features</h2>
 
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===Functional Parameters===
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<partinfo>BBa_K4307000 parameters</partinfo>
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<h2>Characterization </h2>
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<p>The following figure demonstrates our successful construction.</p>
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<div class="center">
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        <div class="thumbinner" style="width:50%;">
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            <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307026-gel.png" class="image">
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                <img alt="" src="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307026-gel.png" width="100%" height=auto class="thumbimage" /></a>                  <div class="thumbcaption">
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                    <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307026-gel.png" class="internal" title="Enlarge"></a>
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                </div>
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                <b>Figure 1: </b> <b>The construction results of PmrA.</b>
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Latest revision as of 16:53, 13 October 2022


PmrA

This part is the transcription factor of Affi-PmrB/A system（BBa_K4307042）, which is modified from the PmrB/A two-component system in E.coli. It is phosphorylated by AffipmrB, then it can activate PmrC promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


Figure 1: The construction results of PmrA.