Difference between revisions of "Part:BBa K4115041:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Add the upstream and downstream fragments of the nitrogen-fixing bacteria (A. caul) nifA gene upstream and downstream of the bleomycin resistance gene. This component was used in the project to edit the nitrogen-fixing bacteria (A. caul) genome by genetic recombination methods.[1]<br> | |
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===Source=== | ===Source=== | ||
− | + | Build via Gibson Assembly. | |
===References=== | ===References=== | ||
+ | [1]Ryu, M. H., Zhang, J., Toth, T., Khokhani, D., Geddes, B. A., Mus, F., Garcia-Costas, A., Peters, J. W., Poole, P. S., Ané, J. M., & Voigt, C. A. (2020). Control of nitrogen fixation in bacteria that associate with cereals. Nature microbiology, 5(2), 314–330. https://doi.org/10.1038/s41564-019-0631-2 |
Latest revision as of 08:16, 4 October 2022
nifA_upstream-BleoR-nifA_downstream
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 434
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1427
Illegal BamHI site found at 1766
Illegal XhoI site found at 547 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 234
Illegal NgoMIV site found at 339
Illegal NgoMIV site found at 556
Illegal NgoMIV site found at 1179
Illegal NgoMIV site found at 1240 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 331
Illegal BsaI.rc site found at 1287
Design Notes
Add the upstream and downstream fragments of the nitrogen-fixing bacteria (A. caul) nifA gene upstream and downstream of the bleomycin resistance gene. This component was used in the project to edit the nitrogen-fixing bacteria (A. caul) genome by genetic recombination methods.[1]
Source
Build via Gibson Assembly.
References
[1]Ryu, M. H., Zhang, J., Toth, T., Khokhani, D., Geddes, B. A., Mus, F., Garcia-Costas, A., Peters, J. W., Poole, P. S., Ané, J. M., & Voigt, C. A. (2020). Control of nitrogen fixation in bacteria that associate with cereals. Nature microbiology, 5(2), 314–330. https://doi.org/10.1038/s41564-019-0631-2