Difference between revisions of "Part:BBa K4119016"

 
 
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<partinfo>BBa_K4119016 short</partinfo>
 
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it's PthlA-FNRbindingsite-7-withRBS
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PthlAF7 was modified based on the PthlA constitutive promoter from Clostridium acetobutyricum.A FNR binding site was inserted at the distance of 7 bp from the - 35 region of the PthlA promoter.
  
  
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4119016 SequenceAndFeatures</partinfo>
 
  
  
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<partinfo>BBa_K4119016 parameters</partinfo>
 
<partinfo>BBa_K4119016 parameters</partinfo>
 
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    <meta http-equiv="X-UA-Compatible" content="IE=edge">
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    <meta name="viewport" content="width=device-width, initial-scale=1.0">
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    <title>Document</title>
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</head>
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<body>
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    <p style="font-size: 180%; font-weight: bold;">Results:</p>
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    <p>The fluorescence intensity shows that the promoter thlF7 has a trends in aerobic inhibition. But the performence in microaerobic situation is also inhibited.</p>
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    <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/img12.png" width="80%" height="80%"></p>
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    <div align="center">
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        <strong>Fig.2. Expression effect of PthlA and PthlAF7-bs2 at different oxygen concentrations</strong>
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    </div>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4119016 SequenceAndFeatures</partinfo>

Latest revision as of 03:58, 14 October 2022


PthlA-FNRbindingsite-7-withRBS

PthlAF7 was modified based on the PthlA constitutive promoter from Clostridium acetobutyricum.A FNR binding site was inserted at the distance of 7 bp from the - 35 region of the PthlA promoter.



Document

Results:

The fluorescence intensity shows that the promoter thlF7 has a trends in aerobic inhibition. But the performence in microaerobic situation is also inhibited.

Fig.2. Expression effect of PthlA and PthlAF7-bs2 at different oxygen concentrations

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]