Difference between revisions of "Part:BBa K4164024"

 
 
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<partinfo>BBa_K4164024 short</partinfo>
 
<partinfo>BBa_K4164024 short</partinfo>
  
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Inductive expression of FXR-ddRFPA1-ddRFPA1-RXR-ddRFPB1-ddRFPB1
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This composite part contains the low intensity promoter (BBa_J23105) and RBS. The composite part can be directly imported into plasmid and express RXRα-CadC.
  
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We recombined low intensity promoter (BBa_J23105) and RBS (BBa_B0034) and RXRα-CadC (BBa_K4164027) by homologous recombination.
  
 
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Latest revision as of 05:56, 12 October 2022


J23105-RXR-Cadc

This composite part contains the low intensity promoter (BBa_J23105) and RBS. The composite part can be directly imported into plasmid and express RXRα-CadC.

We recombined low intensity promoter (BBa_J23105) and RBS (BBa_B0034) and RXRα-CadC (BBa_K4164027) by homologous recombination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1000
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1382