Difference between revisions of "Part:BBa K4165020"
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<partinfo>BBa_K4165020 short</partinfo> | <partinfo>BBa_K4165020 short</partinfo> | ||
| − | This part encodes | + | This part encodes 6x Histidine tag used for purification and characterization of proteins |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | His tag is used for purifying and detecting the recombinant protein without a protein-specific antibody or probe <sup>[1]</sup>. Parts of our systems are fused with his tag for purification and pull-down assay. | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | ===<span class='h3bb'>Sequence and Features</span>=== |
<partinfo>BBa_K4165020 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4165020 SequenceAndFeatures</partinfo> | ||
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| + | ===References=== | ||
| + | 1- Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4. PMID: 26096499. | ||
Latest revision as of 13:30, 10 October 2022
6x Histidine tag
This part encodes 6x Histidine tag used for purification and characterization of proteins
Usage and Biology
His tag is used for purifying and detecting the recombinant protein without a protein-specific antibody or probe [1]. Parts of our systems are fused with his tag for purification and pull-down assay.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1- Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4. PMID: 26096499.