Difference between revisions of "Part:BBa K4134003"
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<partinfo>BBa_K4134003 short</partinfo> | <partinfo>BBa_K4134003 short</partinfo> | ||
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Forward KanR Protein Coding region only. Needs promoter, RBS, and Terminator in order to provide Kan resistance. | Forward KanR Protein Coding region only. Needs promoter, RBS, and Terminator in order to provide Kan resistance. | ||
| − | It helps us to reflect on whether homologous recombination succeeds or not. | + | It helps us to reflect on whether homologous recombination succeeds or not. </p> |
| + | <p> | ||
| + | KanR stands for the Kanamycin resistance gene. We kept the coding region and removed its initiation codon so that KanR shares the same initiation codon with BpfA. Then Kanamycin resistance can be used as the basis to judge whether the strain is recombinant that displays silver-binding protein.</p> | ||
Latest revision as of 14:32, 13 October 2022
KanR
Forward KanR Protein Coding region only. Needs promoter, RBS, and Terminator in order to provide Kan resistance. It helps us to reflect on whether homologous recombination succeeds or not.
KanR stands for the Kanamycin resistance gene. We kept the coding region and removed its initiation codon so that KanR shares the same initiation codon with BpfA. Then Kanamycin resistance can be used as the basis to judge whether the strain is recombinant that displays silver-binding protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]