Difference between revisions of "Part:BBa K4212020"
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<partinfo>BBa_K4212020 short</partinfo> | <partinfo>BBa_K4212020 short</partinfo> | ||
| − | Neutral spacer sequence that separates the coding sequences of Cas9 D15 , and constructs pCas9 level 1 plasmid | + | Neutral spacer sequence that separates the coding sequences of Cas9 and D15 , and constructs pCas9 level 1 plasmid |
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here--> |
| − | === | + | [[File:b20.jpg|600px|thumb|center|Figure 1. The schematic graphic of 0A-Spacer]] |
| + | |||
| + | ===References=== | ||
| + | [1] Garrett, S.C. (2021) Pruning and Tending Immune Memories: Spacer Dynamics in the CRISPR Array. Frontiers in Microbiology. 12. https://www.frontiersin.org/articles/10.3389/fmicb.2021.664299. | ||
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Latest revision as of 19:15, 13 October 2022
0A-Spacer
Neutral spacer sequence that separates the coding sequences of Cas9 and D15 , and constructs pCas9 level 1 plasmid
References
[1] Garrett, S.C. (2021) Pruning and Tending Immune Memories: Spacer Dynamics in the CRISPR Array. Frontiers in Microbiology. 12. https://www.frontiersin.org/articles/10.3389/fmicb.2021.664299.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]