Difference between revisions of "Part:BBa K4345019:Design"

 
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<partinfo>BBa_K4345019 short</partinfo>
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<partinfo>BBa_K4345019 SequenceAndFeatures</partinfo>
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===Design Notes===
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Our team used the RNA thermometer as a part of a killswitch. The sequence was implemented before an antitoxin (ccdB) to regulate its translation based on temperature. It is possible to implement a part of the sequence of the gene that will be translated within the sequence that forms secondary hairpin structure. By doing this, leaky expression will be reduced. Because of the typical short sequence of this thermometer and the position of the startcodon, our team decided not to. The position of the startcodon can be found in the provided figure.
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===Source===
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''E. coli''
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===References===
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Morita, M. T., Tanaka, Y., Kodama, T. S., Kyogoku, Y., Yanagi, H., & Yura, T. (1999, March 15). Translational induction of heat shock transcription factor sigma 32: evidence for a built-in RNA thermosensor. Genes &Amp; Development, 13(6), 655–665. https://doi.org/10.1101/gad.13.6.655

Latest revision as of 14:52, 8 October 2022


RNA Thermometer rpoH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our team used the RNA thermometer as a part of a killswitch. The sequence was implemented before an antitoxin (ccdB) to regulate its translation based on temperature. It is possible to implement a part of the sequence of the gene that will be translated within the sequence that forms secondary hairpin structure. By doing this, leaky expression will be reduced. Because of the typical short sequence of this thermometer and the position of the startcodon, our team decided not to. The position of the startcodon can be found in the provided figure.


Source

E. coli

References

Morita, M. T., Tanaka, Y., Kodama, T. S., Kyogoku, Y., Yanagi, H., & Yura, T. (1999, March 15). Translational induction of heat shock transcription factor sigma 32: evidence for a built-in RNA thermosensor. Genes &Amp; Development, 13(6), 655–665. https://doi.org/10.1101/gad.13.6.655