Difference between revisions of "Part:BBa K4345010"
 
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RNA thermometer used for post-transcriptional regulation based on a temperature-dependent system, based on a RNA thermometer from Listeria monocytogenes. It is maximally expressed at 37ºC and almost not detectable at 30ºC.
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RNA thermometer used for post-transcriptional regulation based on a temperature-dependent system, based on a RNA thermometer from ''Listeria monocytogenes''. It is maximally expressed at 37ºC and almost not detectable at 30ºC. It operates as a reversible molecular zipper that controls the availability of the RBS in its structure. When the temperature increases, the thermometer unzips and the RBS can be detected by the ribosome thus allowing translation of downstream mRNA. When the temperature is below a certain range, the RBS gets trapped (Johansson et al., 2002).
  
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===Usage and Biology===
 
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4345010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4345010 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4345010 parameters</partinfo>
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===Usage and Biology===
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The figure below shows the secondary structure of the RNA thermometer.
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[[Image:PrfA Johansson et al 2002.jpeg|300px]]
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Image obtained from Johansson et al., 2002
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===References===
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Johansson, J., Mandin, P., Renzoni, A., Chiaruttini, C., Springer, M., & Cossart, P. (2002, September). An RNA Thermosensor Controls Expression of Virulence Genes in Listeria monocytogenes. Cell, 110(5), 551–561. https://doi.org/10.1016/s0092-8674(02)00905-4

Latest revision as of 12:58, 8 October 2022


RNA thermometer prfA

RNA thermometer used for post-transcriptional regulation based on a temperature-dependent system, based on a RNA thermometer from Listeria monocytogenes. It is maximally expressed at 37ºC and almost not detectable at 30ºC. It operates as a reversible molecular zipper that controls the availability of the RBS in its structure. When the temperature increases, the thermometer unzips and the RBS can be detected by the ribosome thus allowing translation of downstream mRNA. When the temperature is below a certain range, the RBS gets trapped (Johansson et al., 2002).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

The figure below shows the secondary structure of the RNA thermometer.

PrfA Johansson et al 2002.jpeg

Image obtained from Johansson et al., 2002

References

Johansson, J., Mandin, P., Renzoni, A., Chiaruttini, C., Springer, M., & Cossart, P. (2002, September). An RNA Thermosensor Controls Expression of Virulence Genes in Listeria monocytogenes. Cell, 110(5), 551–561. https://doi.org/10.1016/s0092-8674(02)00905-4