Difference between revisions of "Part:BBa K4425003"
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<partinfo>BBa_K4425003 short</partinfo> | <partinfo>BBa_K4425003 short</partinfo> | ||
− | a polymerase | + | a DNA polymerase that can resist high temperature and perform PCR cycle |
+ | |||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Taq polymerase was assembled into pNEG and pPOS to construct the circuits of positive and negative selection in trp repressor directed evolution.Positive clone in library can lead expression of taq polymerase. Induced Taq polymerase can performed a PCR cycle to replication the sequence of trp repressor | ||
+ | [[Image:25003-1.png|thumbnail|400px|center|'''Figure 1:''' schematic of pNEG and pPOS]] | ||
+ | |||
+ | ==Source== | ||
+ | Taq polymerase come from <i>Thermus aquaticus</i> | ||
− | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4425003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4425003 SequenceAndFeatures</partinfo> |
Latest revision as of 09:30, 6 October 2022
Taq polymerase
a DNA polymerase that can resist high temperature and perform PCR cycle
Usage and Biology
Taq polymerase was assembled into pNEG and pPOS to construct the circuits of positive and negative selection in trp repressor directed evolution.Positive clone in library can lead expression of taq polymerase. Induced Taq polymerase can performed a PCR cycle to replication the sequence of trp repressor
Source
Taq polymerase come from Thermus aquaticus
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1591
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2037
Illegal PstI site found at 1591 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1772
Illegal XhoI site found at 325
Illegal XhoI site found at 1402
Illegal XhoI site found at 2359 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1591
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1591
Illegal NgoMIV site found at 1426 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1915
Illegal BsaI.rc site found at 2155
Illegal SapI site found at 1227
Illegal SapI site found at 2295
Illegal SapI.rc site found at 1617
Illegal SapI.rc site found at 2205