Difference between revisions of "Part:BBa K4488007:Design"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K4488001 short</partinfo>
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<partinfo>BBa_K4488007 short</partinfo>
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<partinfo>BBa_K4488001 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4488007 SequenceAndFeatures</partinfo>
 
===Design Notes===
 
===Design Notes===
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Some commonly used restriction sites in the orginal sequence have been removed.
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The design of the part was based on the sfGFP-CBD fusion protein (BBa_K1321341) developed by the 2014 Imperial team Aqualose. The fuGFP sequence can be found here (BBa_K3814004).
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The construct has a constitutive RBS (BBa_B0034). 6bp cloning scar (“accgcc”) was also inserted between the fuGFP and CBDclos, similar to BBa_K1321341, to act as a small linker.
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===Source===
 
===Source===
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GenBank: AIF28002.1. https://doi.org/10.1007/s11033-014-3575-x
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The design of the part was based on the sfGFP-CBD fusion protein (BBa_K1321341) developed by the 2014 Imperial team Aqualose with the type of GFP replaced. The fuGFP sequence can be found here (BBa_K3814004).
 +
 
 
===References===
 
===References===

Latest revision as of 04:58, 9 October 2022

Fusion of free-use GFP with CBDclos (cellulose-binding domain) at the C-terminal end


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The design of the part was based on the sfGFP-CBD fusion protein (BBa_K1321341) developed by the 2014 Imperial team Aqualose. The fuGFP sequence can be found here (BBa_K3814004). The construct has a constitutive RBS (BBa_B0034). 6bp cloning scar (“accgcc”) was also inserted between the fuGFP and CBDclos, similar to BBa_K1321341, to act as a small linker.

Source

The design of the part was based on the sfGFP-CBD fusion protein (BBa_K1321341) developed by the 2014 Imperial team Aqualose with the type of GFP replaced. The fuGFP sequence can be found here (BBa_K3814004).

References