Difference between revisions of "Part:BBa K4488000"

 
 
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<partinfo>BBa_K4488000 short</partinfo>
 
<partinfo>BBa_K4488000 short</partinfo>
  
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Intimin is an adhesion protein of EHEC. The N-terminus of beta-intimin (Neae) has been used for surface display in E. coli, specifically for sdAbs/Nanobodies. Neae consists of a trans-membrane beta barrel and requires the BAM complex for activation.  
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Intimin is an adhesion protein of EHEC. The N-terminus of beta-intimin (Neae) has been used for surface display in E. coli, specifically for sdAbs/Nanobodies. Neae consists of a trans-membrane beta barrel and requires the BAM complex for activation. 6000-8000 molecules of Neae are expressed per cell (Salema et.al., 2013).  
  
 
Reference:  
 
Reference:  
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https://doi.org/10.1111%2F1751-7915.12819
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Salema, V., E. Marín, R. Martínez-Arteaga, D. Ruano-Gallego, S. Fraile, Y. Margolles, X. Teira, C. Gutierrez, G. Bodelón, and LÁ Fernández. 2013. 'Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies', PLoS One, 8: e75126.
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Salema, V., and LÁ Fernández. 2017. 'Escherichia coli surface display for the selection of nanobodies', Microb Biotechnol, 10: 1468-84.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 07:26, 12 October 2022


Neae: N terminus of 𝛃-intimin for Surface Display in E. coli

Intimin is an adhesion protein of EHEC. The N-terminus of beta-intimin (Neae) has been used for surface display in E. coli, specifically for sdAbs/Nanobodies. Neae consists of a trans-membrane beta barrel and requires the BAM complex for activation. 6000-8000 molecules of Neae are expressed per cell (Salema et.al., 2013).

Reference: Salema, V., E. Marín, R. Martínez-Arteaga, D. Ruano-Gallego, S. Fraile, Y. Margolles, X. Teira, C. Gutierrez, G. Bodelón, and LÁ Fernández. 2013. 'Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies', PLoS One, 8: e75126.

Salema, V., and LÁ Fernández. 2017. 'Escherichia coli surface display for the selection of nanobodies', Microb Biotechnol, 10: 1468-84.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]