Difference between revisions of "Part:BBa K4417015"

 
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4417015 short</partinfo>
 
<partinfo>BBa_K4417015 short</partinfo>
  
−
aa
+
<h1>Description</h1>
 +
 
 +
This part is the coding sequence of ureEFG accessory proteins from Sporosarcina pasteurii. It was cloned into the master plasmid (BBa_K4417017).
 +
 
 +
This part is not Type IIS compatible as it has BsaI sites.
 +
 
 +
[[File:Adkjb.png|600px|thumb|center|'''Figure 1:''' Basic part ureEFG TU2B]]
 +
 
 +
<h1>Usage and Biology</h1>
 +
 
 +
*  This basic part can be used to produce urease accessory proteins.
 +
*  It can be cloned into the master plasmid (BBa_K4417017) to express the nature urease operon from Sporosarcina pasteurii using Golden Gate assembly.
 +
*  The part was synthesized by IDT as a gBlock.
 +
 
 +
<h1>Reference</h1>
 +
 
 +
1. Zerner, B. “Recent advances in the chemistry of an old enzyme, urease.” Bioorg. Chem. 19 (1991):116-131
 +
 
 +
2. Krajewska, Barbara. "Ureases I. Functional, catalytic and kinetic properties: A review". Journal of Molecular Catalysis B: Enzymatic 59 no.1-3 (2009):9–21. doi:10.1016/j.molcatb.2009.01.003
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:25, 12 October 2022


CuO-RBS-ureABC-rrnB T1 Terminator TU1B

Description

This part is the coding sequence of ureEFG accessory proteins from Sporosarcina pasteurii. It was cloned into the master plasmid (BBa_K4417017).

This part is not Type IIS compatible as it has BsaI sites.

Figure 1: Basic part ureEFG TU2B

Usage and Biology

  • This basic part can be used to produce urease accessory proteins.
  • It can be cloned into the master plasmid (BBa_K4417017) to express the nature urease operon from Sporosarcina pasteurii using Golden Gate assembly.
  • The part was synthesized by IDT as a gBlock.

Reference

1. Zerner, B. “Recent advances in the chemistry of an old enzyme, urease.” Bioorg. Chem. 19 (1991):116-131

2. Krajewska, Barbara. "Ureases I. Functional, catalytic and kinetic properties: A review". Journal of Molecular Catalysis B: Enzymatic 59 no.1-3 (2009):9–21. doi:10.1016/j.molcatb.2009.01.003

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 969
    Illegal BamHI site found at 1
    Illegal XhoI site found at 1723
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 2066