Difference between revisions of "Part:BBa K4344044"

 
 
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<partinfo>BBa_K4344044 short</partinfo>
 
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Plasmid used for recombinant expression of UL19 His tag protein in HeLa cells. Also used for qPCR plasmid titration assay. For further information see wiki https://2022.igem.wiki/heidelberg/experiments-sirna.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4344044 SequenceAndFeatures</partinfo>
 
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[[File: PRK5-HSV UL19 AA816 1148.png|thumbnail|center|500px|<b>Figure 1: Proposed plasmid map of pRK5-HSV <i>UL19</i> AA816:1148-6xHis</b> ]]
  
  

Latest revision as of 14:41, 12 October 2022


pRK5-HSV UL19 6xHis

Plasmid used for recombinant expression of UL19 His tag protein in HeLa cells. Also used for qPCR plasmid titration assay. For further information see wiki https://2022.igem.wiki/heidelberg/experiments-sirna.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 919
    Illegal SpeI site found at 28
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 919
    Illegal SpeI site found at 28
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 919
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 919
    Illegal SpeI site found at 28
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 919
    Illegal SpeI site found at 28
    Illegal NgoMIV site found at 1231
    Illegal NgoMIV site found at 1378
    Illegal NgoMIV site found at 2905
  • 1000
    COMPATIBLE WITH RFC[1000]
Figure 1: Proposed plasmid map of pRK5-HSV UL19 AA816:1148-6xHis