Difference between revisions of "Part:BBa K257005:Design"

(Design Notes)
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===Design Notes===
 
===Design Notes===
  
Product created by PCR amplification
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Product created by PCR amplification. For fusion of proteins to the N-terminal extremity of TolRII using the silver fusion standard RFC23. The suffix used to create this parts conform to the std 10 guidelines for coding DNA.
 
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This part is inserted in the topo vector.
 
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Molecule tolR
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Forward primer (tolR II Fusion Fw) bp pos 130
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Reverse primer (tolR II Rw) bp pos 351
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===Source===
 
===Source===
  
genomic DNA
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Sequence from E. coli k 12 strain MG1655 (refseq accessor NC_000913.2)
  
 
===References===
 
===References===

Latest revision as of 15:58, 21 October 2009

TolR domain II N-term fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Product created by PCR amplification. For fusion of proteins to the N-terminal extremity of TolRII using the silver fusion standard RFC23. The suffix used to create this parts conform to the std 10 guidelines for coding DNA. This part is inserted in the topo vector.

Source

Sequence from E. coli k 12 strain MG1655 (refseq accessor NC_000913.2)

References