Difference between revisions of "Part:BBa K257003:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K257003 short</partinfo>
 
<partinfo>BBa_K257003 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Enzymatic restrictions sites surrounded the RBS-TetR
 
  
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* In our project we use g3p as a fusion to OmpA-Linker (BBa_K103996) which need SacI restriction site for inframe fusion.
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* So we design g3p with SacI site at the N-terminal. SacI (GAGCT^C) site is shared with XbaI (T^CTAGA) in order to have SacI site for fusion and standard sites.
  
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The prefix used to create this parts conform to the std 10 guidelines for coding DNA.
  
 
===Source===
 
===Source===
  
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plasmid
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Standardisation of [https://parts.igem.org/Part:BBa_K103006 BBa_K103006] from Warshaw 2008.
  
 
===References===
 
===References===

Latest revision as of 15:54, 21 October 2009

Outer membrane protein A (partial) fused to linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • In our project we use g3p as a fusion to OmpA-Linker (BBa_K103996) which need SacI restriction site for inframe fusion.
  • So we design g3p with SacI site at the N-terminal. SacI (GAGCT^C) site is shared with XbaI (T^CTAGA) in order to have SacI site for fusion and standard sites.

The prefix used to create this parts conform to the std 10 guidelines for coding DNA.

Source

Standardisation of BBa_K103006 from Warshaw 2008.

References