Difference between revisions of "Part:BBa K4256166:Design"

 
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===Source===
 
===Source===
  
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It comes from the combination of composite parts BBa_K4256168, BBa_K4256108, BBa_K4256106, BBa_K4256122 and BBa_K42561886, and the source of each part can be checked.  
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It comes from the combination of composite parts BBa_I712074, BBa_K1222999, BBa_K3716001, BBa_K4256122 and BBa_B0012, and the source of each part can be checked.
  
 
===References===
 
===References===

Latest revision as of 17:36, 11 October 2022


ccdB toxin expression system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 158
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 119
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 158
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 158
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BamHI is added before the ccdB sequence while HindIII is added after the ccdB sequence to facilitate separate vector concatenation and enzymatic identification recovery afterward, but there are no these two enzymatic sites in the whole sequence. The uploaded BBa_K4256166 sequence contains a lactose manipulator-related sequence to facilitate IPTG induction during experiments, but the full sequence for practical applications does not contain a lac operon or can be enzymatically excised away.


Source

It comes from the combination of composite parts BBa_I712074, BBa_K1222999, BBa_K3716001, BBa_K4256122 and BBa_B0012, and the source of each part can be checked.

References