Difference between revisions of "Part:BBa K4193012:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | kill switch | + | the kill switch contains YopE mRNA, which is a cytotoxin form Yersinia, and a ribozyme riboswitch. When no target molecule binds to the aptamer sequence, YopE is expressed and kills the cell, whereas the hammerhead ribozyme sequence degrades YopE mRNA. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | synthesize from company | |
===References=== | ===References=== | ||
| + | Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104. | ||
Latest revision as of 10:18, 8 October 2022
kill switch
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 385
Illegal PstI site found at 698 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 385
Illegal PstI site found at 698 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 661
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 385
Illegal PstI site found at 698 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 385
Illegal PstI site found at 698
Illegal AgeI site found at 252 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
the kill switch contains YopE mRNA, which is a cytotoxin form Yersinia, and a ribozyme riboswitch. When no target molecule binds to the aptamer sequence, YopE is expressed and kills the cell, whereas the hammerhead ribozyme sequence degrades YopE mRNA.
Source
synthesize from company
References
Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104.