Difference between revisions of "Part:BBa K4321004"
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<partinfo>BBa_K4321004 short</partinfo> | <partinfo>BBa_K4321004 short</partinfo> | ||
− | RFP | + | mScarlet is a red fluorescent protein (RFP) which was engineered in 2017 and based on a synthetic gene template consisting of mCherry, chromo proteins, and naturally occurring RFPs. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This fluorescent protein carries the highest brightness in the RFP class and a fluorescent life of 3.9 nanoseconds. mScarlet has an absorbance of 569 nanometers and emission maxima of 594 nanometers. | ||
− | + | ===Sequence and Features=== | |
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<partinfo>BBa_K4321004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4321004 SequenceAndFeatures</partinfo> | ||
+ | ==References== | ||
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+ | Bindels, D., Haarbosch, L., van Weeren, L. et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods 14, 53–56 (2017). https://doi.org/10.1038/nmeth.4074 | ||
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+ | mScarlet . SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=mScarlet | ||
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Latest revision as of 21:04, 9 October 2022
mScarlet
mScarlet is a red fluorescent protein (RFP) which was engineered in 2017 and based on a synthetic gene template consisting of mCherry, chromo proteins, and naturally occurring RFPs.
Usage and Biology
This fluorescent protein carries the highest brightness in the RFP class and a fluorescent life of 3.9 nanoseconds. mScarlet has an absorbance of 569 nanometers and emission maxima of 594 nanometers.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 512
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Bindels, D., Haarbosch, L., van Weeren, L. et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods 14, 53–56 (2017). https://doi.org/10.1038/nmeth.4074
mScarlet . SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=mScarlet