Difference between revisions of "Part:BBa K4321001"

 
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<partinfo>BBa_K4321001 short</partinfo>
 
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Blue variant of Aequorea victoria GFP. Mammalian codon-optimized.
 
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===Usage and Biology===
 
===Usage and Biology===
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This blue fluorescent protein (BFP) one of the 19 variants of Aequorea victoria derived green fluorescent protein (GFP). This BFP differs from its predecessor in that it contains a tyrosine (Y) to histidine (H) substitution at position 66. This Y66H substitution results in the board excitation close to 380 namometers and emission at 448 namometers. This BFP variant carries an additional modification, the addition of a mammalian codon directly downstream of the start methionin allowing its expression in mammalian cells.
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4321001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4321001 SequenceAndFeatures</partinfo>
  
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==Reference==
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BFP sequence and map. SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=BFP
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Education in microscopy and digital imaging. ZEISS Microscopy Online Campus | Jellyfish Fluorescent Proteins. (n.d.). Retrieved October 4, 2022, from https://zeiss-campus.magnet.fsu.edu/articles/probes/jellyfishfps.html
  
 
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Latest revision as of 22:06, 7 October 2022


BFP

Usage and Biology

This blue fluorescent protein (BFP) one of the 19 variants of Aequorea victoria derived green fluorescent protein (GFP). This BFP differs from its predecessor in that it contains a tyrosine (Y) to histidine (H) substitution at position 66. This Y66H substitution results in the board excitation close to 380 namometers and emission at 448 namometers. This BFP variant carries an additional modification, the addition of a mammalian codon directly downstream of the start methionin allowing its expression in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

BFP sequence and map. SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=BFP

Education in microscopy and digital imaging. ZEISS Microscopy Online Campus | Jellyfish Fluorescent Proteins. (n.d.). Retrieved October 4, 2022, from https://zeiss-campus.magnet.fsu.edu/articles/probes/jellyfishfps.html