Difference between revisions of "Part:BBa K4152001:Design"

 
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===Design Notes===
 
===Design Notes===
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To improve the stability of wild-type proteinase K, we designed 2 mutation sites: T16C-N257C.
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To improve the performance of Proteinase K, we designed many Proteinase K mutant genes. PK_MT1 is a complicated mutation of Proteinase K, which contains 5 mutation sites: N257C-S173Y-T16C-T20D-V198F.
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===Source===
 
===Source===

Latest revision as of 17:35, 28 September 2022


PK_MT1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 235
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 235
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To improve the performance of Proteinase K, we designed many Proteinase K mutant genes. PK_MT1 is a complicated mutation of Proteinase K, which contains 5 mutation sites: N257C-S173Y-T16C-T20D-V198F.

Source

We make multiple site mutations on the basis of a serine protease proteinase K from Tritirachium album limber. (PDB ID: 1ic6)

References