Difference between revisions of "Part:BBa K4329001"
 
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===What is it?===
 
===What is it?===
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In order to alter the metabolic pathway of E. coli and tend to a more productive way of Biotin, we enhance the metabolic pathway in the E. coli by increasing the expression of desthiobiotin synthase and biotin synthase. This plasmid is regulated by the T7 promoter, Lac operator, and T7 terminator.   
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In order to alter the metabolic pathway of E. coli and tend to a more productive way of biotin, we enhance the metabolic pathway in the E. coli by increasing the expression of dethiobiotin synthase and biotin synthase. This plasmid is regulated by the T7 promoter, Lac operator, and T7 terminator.   
  
 
===Usage and Biology===
 
===Usage and Biology===
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[[File:Dethiobiotin expression.png|700px|thumb|center|Fg.1 pathway of increasing dethiobiotin synthase and biotin synthase]]
  
 
With IPTG, RNA ligase will converge with the T7 promoter, leading to mRNA production. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating an ample amount of biotin.
 
With IPTG, RNA ligase will converge with the T7 promoter, leading to mRNA production. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating an ample amount of biotin.
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===Characterization===
 
===Characterization===
  
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[[File:001 result.png|700px|thumb|center| Fig. 2 Increasing expression of dethiobiotin synthase and biotin synthase can enhance E. coli’s productivity  of biotin. (A)Primers designed for dethiobiotin synthase and biotin synthase. (B)Electrophoresis of dethiobiotin synthase and biotin synthase.]]
  
 
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Latest revision as of 11:57, 3 October 2022


Overexpression of dethiobiotin synthase and biotin synthase in E. coli

What is it?

In order to alter the metabolic pathway of E. coli and tend to a more productive way of biotin, we enhance the metabolic pathway in the E. coli by increasing the expression of dethiobiotin synthase and biotin synthase. This plasmid is regulated by the T7 promoter, Lac operator, and T7 terminator.

Usage and Biology

Fg.1 pathway of increasing dethiobiotin synthase and biotin synthase

With IPTG, RNA ligase will converge with the T7 promoter, leading to mRNA production. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating an ample amount of biotin.

Characterization

Fig. 2 Increasing expression of dethiobiotin synthase and biotin synthase can enhance E. coli’s productivity of biotin. (A)Primers designed for dethiobiotin synthase and biotin synthase. (B)Electrophoresis of dethiobiotin synthase and biotin synthase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]