Difference between revisions of "Part:BBa K4387997:Design"

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===Design Notes===
 
===Design Notes===
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Shortening this sequence to the essential last 60 amino acids has not been tested yet.
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Fusion of the HlyA-tag to a protein allows its secretion with the one-step hemolysin A secretion machinery. The composite part <partinfo>BBa_K4387987</partinfo> contains all the necessary BioBricks for the constitutive expression of the secretion system.
−
 
+
Shortening this sequence to the essential last 60 amino acids has not been tested yet for its functionality.
  
  
 
===Source===
 
===Source===
 +
Sequence of the HlyA-tag was obtain from the paper of Ruano-Gallego et al. [1]
  
−
Sequence of the HlyD was obtain from the paper of Ruano-Gallego et al. [1]
 
  
 
===References===
 
===References===
 
[1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0
 
[1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0

Latest revision as of 10:46, 6 October 2022


HlyA-tag obtained from the hemolysin A one-step secretion system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fusion of the HlyA-tag to a protein allows its secretion with the one-step hemolysin A secretion machinery. The composite part BBa_K4387987 contains all the necessary BioBricks for the constitutive expression of the secretion system. Shortening this sequence to the essential last 60 amino acids has not been tested yet for its functionality.


Source

Sequence of the HlyA-tag was obtain from the paper of Ruano-Gallego et al. [1]


References

[1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0