Difference between revisions of "Part:BBa K3743019"
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<partinfo>BBa_K4140014 short</partinfo>
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Complementary part to crRNA present down stream to the target gene  
 
Complementary part to crRNA present down stream to the target gene  
 
==Usage==
 
==Usage==
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we employed this part to attract our regulatory system Cas12g guided by specific RNA  to PAH mRNA that we want to stop its translation in case of overexpression of PAH  or high levels OF tyrosine.
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==Literature Characterization==
 
==Literature Characterization==
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4140014 parameters</partinfo>
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<partinfo>BBa_K3743019 parameters</partinfo>
 
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Latest revision as of 09:52, 29 September 2022


gRNA Binding site


Part Description

Complementary part to crRNA present down stream to the target gene

Usage

we employed this part to attract our regulatory system Cas12g guided by specific RNA to PAH mRNA that we want to stop its translation in case of overexpression of PAH or high levels OF tyrosine.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]