Difference between revisions of "Part:BBa K4324001:Design"

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<partinfo>BBa_K4324001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4324001 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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The protein sequence was codon-optimised for Escherichia coli through the [https://sg.idtdna.com/CodonOpt IDT codon optimisation tool] as gBlocks Gene Fragments.
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The protein sequence was codon-optimised for expression in '''Escherichia coli K12''' through the [https://sg.idtdna.com/CodonOpt IDT codon optimisation tool] as gBlocks® Gene Fragments, and ensured that EcoRI, XbaI, SpeI, and PstI restriction sites were not accidentally present within the CDS.
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===Source===
 
===Source===
  
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LacPO:
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This original protein sequence was taken from the '''XYL2 gene''' of '''Saccharomyces cerevisiae''' (also known as '''Baker's yeast''') (strain ATCC 204508 / S288c), which can be found on the [https://rest.uniprot.org/uniprotkb/Q07993.fasta Uniprot database].
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- RBS:  
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- CDS: XYL2 gene from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Taken from the [https://www.uniprot.org/uniprotkb/Q07993/entry Uniprot database].
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Alternatively, the protein sequence is pasted below:
  
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- Terminator:
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<code>
 +
MTDLTTQEAIVLERPGKITLTNVSIPKISDPNEVIIQIKATGICGSDIHYYTHGRIANYV
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VESPMVLGHESSGIVALIGENVKTLKVGDRVALEPGIPDRFSPEMKEGRYNLDPNLKFAA
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TPPFDGTLTKYYKTMKDFVYKLPDDVSFEEGALIEPLSVAIHANKLAKIKFGARCVVFGA
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GPIGLLAGKVASVFGAADVVFVDLLENKLETARQFGATHIVNSGDLPHGVTVDSVIKKAI
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GKKGADVVFECSGAEPCVRAGIEVCKAGGTIVQVGMGQEEIQFPISIIPTKELTFQGCFR<br>
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YCQGDYSDSIELVSSRKLSLKPFITHRYSFKDAVEAFEETSHHPLNNIKTIIEGPE
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</code>
  
 
===References===
 
===References===
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1. https://www.uniprot.org/uniprotkb/Q07993/entry<br>
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2. https://www.genome.jp/dbget-bin/www_bget?ec:1.1.1.9

Latest revision as of 14:12, 12 October 2022


lacPO-RBS-XDH-T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 700
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 357

Design Notes

The protein sequence was codon-optimised for expression in Escherichia coli K12 through the IDT codon optimisation tool as gBlocks® Gene Fragments, and ensured that EcoRI, XbaI, SpeI, and PstI restriction sites were not accidentally present within the CDS.

Source

This original protein sequence was taken from the XYL2 gene of Saccharomyces cerevisiae (also known as Baker's yeast) (strain ATCC 204508 / S288c), which can be found on the Uniprot database.

Alternatively, the protein sequence is pasted below:

MTDLTTQEAIVLERPGKITLTNVSIPKISDPNEVIIQIKATGICGSDIHYYTHGRIANYV VESPMVLGHESSGIVALIGENVKTLKVGDRVALEPGIPDRFSPEMKEGRYNLDPNLKFAA TPPFDGTLTKYYKTMKDFVYKLPDDVSFEEGALIEPLSVAIHANKLAKIKFGARCVVFGA GPIGLLAGKVASVFGAADVVFVDLLENKLETARQFGATHIVNSGDLPHGVTVDSVIKKAI GKKGADVVFECSGAEPCVRAGIEVCKAGGTIVQVGMGQEEIQFPISIIPTKELTFQGCFR
YCQGDYSDSIELVSSRKLSLKPFITHRYSFKDAVEAFEETSHHPLNNIKTIIEGPE

References

1. https://www.uniprot.org/uniprotkb/Q07993/entry
2. https://www.genome.jp/dbget-bin/www_bget?ec:1.1.1.9